NC 009779

   LOCUS       NC_009779              31208 bp    DNA     circular BCT 07-SEP-2007
   DEFINITION  Enterobacter sakazakii ATCC BAA-894 plasmid pESA2, complete
               sequence.
   ACCESSION   NC_009779
   VERSION     NC_009779.1  GI:156936507
   KEYWORDS    .
   SOURCE      Enterobacter sakazakii ATCC BAA-894
     ORGANISM  Enterobacter sakazakii ATCC BAA-894
               Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
               Enterobacteriaceae; Cronobacter.
   REFERENCE   1  (bases 1 to 31208)
     CONSRTM   NCBI Genome Project
     TITLE     Direct Submission
     JOURNAL   Submitted (06-SEP-2007) National Center for Biotechnology
               Information, NIH, Bethesda, MD 20894, USA
   REFERENCE   2  (bases 1 to 31208)
     AUTHORS   McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J.,
               Clifton,W.S., Latreille,P., Courtney,L., Pepin,K., Bhonagiri,V.,
               Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R.
     CONSRTM   The Enterobacter sakazakii Genome Sequencing Project
     TITLE     Direct Submission
     JOURNAL   Submitted (25-JUL-2007) Genetics, Genome Sequencing Center, 4444
               Forest Park Parkway, St. Louis, MO 63108, USA
   COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
               NCBI review. The reference sequence was derived from CP000784.
               E. sakazakii--Enterobacter sakazakii is rarely encountered in
               clinical specimens, and is more prevalent in the environment and in
               food. However, Enterobacter sakazakii is strongly implicated in
               food borne diseases causing severe meningitis or enteritis,
               especially in neonates and infants (Nazarowec-White and Farber, Int
               J FoodMicrobiol. 1997 Feb;34(2):103-13).
               
               The strain of Enterobacter sakazakii being sequenced was isolated
               from powdered milk formula fed to a hospitalized neonate that
               developed an infection (Centers for Disease Control and
               Prevention). It is available from the American Type Culture
               Collection as ATCC BAA-894 or from the Salmonella Genetic Stock
               Centre as SGSC4695. The genome was sequenced to 8X coverage, using
               plasmid and fosmid libraries, and was finished to an error rate of
               less than 1 per 10,000 bases. Automated annotation was performed
               and manual annotation will continue in the labs of Michael
               McClelland and Kenneth Sanderson. The National Institute of Allergy
               and Infectious Diseases (NIAID), National Institutes of Health
               (NIH) has funded this project.
               
               Coding sequences below are predicted using GeneMark v3.3 and
               Glimmer2  v2.13. Intergenic regions not spanned by GeneMark and
               Glimmer2 were blasted against NCBI's non-redundant (NR) database
               and predictions generated based on protein alignments. RNA genes
               were determined  using tRNAscan-SE 1.23 or Rfam v8.0. This sequence
               was finished as follows unless otherwise noted: all regions were
               double stranded, sequenced with an alternate chemistries or covered
               by high quality data(i.e., phred quality >=30);an attempt was made
               to resolve all sequencing problems, such as compressions and
               repeats; all regionswere covered by sequence from more than one m13
               subclone.
               COMPLETENESS: full length.